Location:

City:
Directors:

Website:

The Centre for Applied Genomics (TCAG),
Hospital for Sick Children
Toronto, Ontario
Jayne Danska and Steve Scherer

http://tcag.bioinfo.sickkids.on.ca/

Applications for mouse molecular cytogenetic technologies include: mouse modeling of genetic instability syndromes, identification of somatic chromosomal aberrations in pre-neoplastic and tumor cells, analyses of skin, neuronal and embryonic stem cells critical to development of future cell-based clinical interventions, localization of transgenic genome inserts, and quality control of ES cell lines for genome modification. Our objective is to provide CGDN and other Canadian researchers with economical access to a full complement of cytogenetic and genomic approaches tailored to their research problem with a high standard of quality.

Investigators supply healthy cultured cells suitable for colcemid arrest and fixation within 24 hours of receipt, and will be notified when images have been collected and chromosomes identified.

• provision of cloned probes
• single/dual color FISH and (multicolor) m-FISH experiments
• image analysis and assistance with data interpretation
• training in metaphase spread preparation, image collection and analysis for heavy users
• follow-up studies including real-time PCR to quantify gene dosage alterations, and comparative genomic hybridization (CGH), on tiled BAC arrays

Cost: Single FISH $200 for CGDN members ($260 for non-members), Double FISH $300 for CGDN members ($390 for non-members), M-FISH $400 ($520 for non-members). *Additional work on data analysis or extensive data collection will be billed separately.